DNA methylation protocols /
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作 者：edited by Ken I. Mills and Bernard H. Ramsahoye.
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ISBN：9780896036185
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Summary:
Publisher Summary 1
Methylation is an integral part of DNA replication and transcription. In this reference for researchers, 14 contributions from international scientists discuss new techniques for the study of methylation at the global level and the level of sequence. Methods explained include those used in identifying aberrantly methylated genes related to cancer and aging. Each technique described includes a summary of the basic theory behind it, a complete materials list, and step-by-step instructions. Annotation c. Book News, Inc., Portland, OR (booknews.com)
目录
Preface p. v
Contributors p. ix
1 Overview Ken I. Mills and Bernard H. Ramsahoye p. 1
2 Nearest-Neighbor Analysis Bernard H. Ramsahoye p. 9
3 Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography Bernard H. Ramsahoye p. 17
4 Methylation Analysis by Chemical DNA Sequencing Piroska E. Szabo and Jeffrey R. Mann and Gerd P. Pfeifer p. 29
5 Methylation-Sensitive Restriction Fingerprinting Catherine S. Davies p. 43
6 Restriction Landmark Genome Scanning Joseph F. Costello and Christoph Plass and Webster K. Cavenee p. 53
7 Combined Bisulfite Restriction Analysis (COBRA) Cindy A. Eads and Peter W. Laird p. 71
8 Differential Methylation Hybridization Using CpG Island Arrays Pearlly S. Yan and Susan H. Wei and Tim Hui-Ming Huang p. 87
9 Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences Minoru Toyota and Jean-Pierre J. Issa p. 101
10 Isolation of CpG Islands Using a Methyl-CpG Binding Column Sally H. Cross p. 111
11 Purification of MeCP2-Containing Deacetylase from Xenopus laevis Peter L. Jones and Paul A. Wade and Alan P. Wolffe p. 131
12 DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method Petra Hajkova and Osman El-Maarri and Sabine Engemann and Joachim Oswald and Alexander Olek and Jorn Walter p. 143
13 Measuring DNA Demethylase Activity In Vitro Moshe Szyf and Sanjoy K. Bhattacharya p. 155
14 Extracting DNA Demethylase Activity from Mammalian Cells Moshe Szyf and Sanjoy K. Bhattacharya p. 163
Index p. 177
Contributors p. ix
1 Overview Ken I. Mills and Bernard H. Ramsahoye p. 1
2 Nearest-Neighbor Analysis Bernard H. Ramsahoye p. 9
3 Measurement of Genome-Wide DNA Cytosine-5 Methylation by Reversed-Phase High-Pressure Liquid Chromatography Bernard H. Ramsahoye p. 17
4 Methylation Analysis by Chemical DNA Sequencing Piroska E. Szabo and Jeffrey R. Mann and Gerd P. Pfeifer p. 29
5 Methylation-Sensitive Restriction Fingerprinting Catherine S. Davies p. 43
6 Restriction Landmark Genome Scanning Joseph F. Costello and Christoph Plass and Webster K. Cavenee p. 53
7 Combined Bisulfite Restriction Analysis (COBRA) Cindy A. Eads and Peter W. Laird p. 71
8 Differential Methylation Hybridization Using CpG Island Arrays Pearlly S. Yan and Susan H. Wei and Tim Hui-Ming Huang p. 87
9 Methylated CpG Island Amplification for Methylation Analysis and Cloning Differentially Methylated Sequences Minoru Toyota and Jean-Pierre J. Issa p. 101
10 Isolation of CpG Islands Using a Methyl-CpG Binding Column Sally H. Cross p. 111
11 Purification of MeCP2-Containing Deacetylase from Xenopus laevis Peter L. Jones and Paul A. Wade and Alan P. Wolffe p. 131
12 DNA-Methylation Analysis by the Bisulfite-Assisted Genomic Sequencing Method Petra Hajkova and Osman El-Maarri and Sabine Engemann and Joachim Oswald and Alexander Olek and Jorn Walter p. 143
13 Measuring DNA Demethylase Activity In Vitro Moshe Szyf and Sanjoy K. Bhattacharya p. 155
14 Extracting DNA Demethylase Activity from Mammalian Cells Moshe Szyf and Sanjoy K. Bhattacharya p. 163
Index p. 177
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