英汉对照分子生物学导论（[美]西尔维恩·W.勒潘)（第二版）

Introduction

Chapter 1 Amino Acids to Proteins

1.1Protein Composition

1.2Protein Conformations

1.2.1Describing Protein Structure

1.2.2Chemical and Physical Basis for Protein Folding

1.3Protein Structure and Function:A Few Examples

1.4The Dynamics of Proteins

1.5Experiments

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 2 Nucleic Acids

2.1Properties of a Genetic Material

2.2Nucleic Acids and DNA structure

2.2.1Nucleotides

2.2.2General Structure of Nucleic Acids

2.2.3Structure of DNA

2.3DNA as the Genetic Material

2.4DNA in the Cell

2.5RNA (Ribonucleic Acid)

2.6Experiments

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 3 Transcription inProkaryotes:Mechanism and Regulation

3.1Why Use an RNA Intermediate

3.2Mechanism of Transcription

3.2.1Promoters

3.2.2RNA Polymerase

3.2.3Transcription Mechanism in Three Phases

3.3Regulation of Gene Expression in Prokaryotes

3.3.1Coordinate Regulation

3.3.2The Lac Operon

3.3.3The Trp Operon

3.3.4Ara and Gal Operons

3.4Experiments

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 4 Transcription inEukaryotes:Mechanism and Regulation

4.1Eukaryotic RNA Polymerases

4.2Eukaryotic Promoters

4.3General Transcription Factors and Initiation

4.3.1TFⅡD

4.3.2Other TFⅡs

4.3.3General Transcription Factors for RNA Polymerase Ⅰand Ⅲ

4.4Specific Transcription Factors and Transcriptional Regulation

4.4.1Activators

4.4.2Repressors

4.4.3Enhancers and Silencers

4.5Structures of Specific Transcription Factors

4.5.1DNA-Binding Motifs in Prokaryotes

4.5.2DNA-Binding Motifs in Eukaryotes

4.6Experiments

4.6.1RNA Polymerase Targets

4.6.2Modularity of Specific Transcription Factors

Vocabulary

Review Questions

Exploration Questions



Chapter 5 mRNA Modifications inEukaryotes

5.1Capping

5.2Polyadenylation

5.3Splicing

5.3.1The Basic Splicing Reaction

5.3.2Proteins Involved in Splicing

5.3.3Self-Splicing

5.3.4Trans-Splicing

5.3.5Reasons for Introns

5.4mRNA Editing

5.5Experiments

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 6 Translation

6.1The Genetic Code

6.2Mechanism of Translation in Prokaryotes

6.2.1Initiation

6.2.2Elongation

6.2.3Termination

6.3Translation in Eukaryotes

6.4tRNA Structure and Wobble

6.4.1Anti-codons

6.4.2Wobble

6.5Experiments

6.5.1Deciphering the Genetic Code

6.5.2Direction of Translation

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 7 Regulation of Gene Expressionin Eukaryotes

7.1Histones and Transcriptional Regulation

7.1.1Histones and DNA Organization

7.1.2Histones and Transcription

7.1.3Covalent Modification of Histones

7.1.4Proteins that Recognize and Modify Histones

7.2Post-Transcriptional Regulation

7.3Nuclear Export

7.4RNA Stability

7.4.1mRNA Stability Regulation by Proteins

7.4.2mRNA Stability Regulation by Small RNAs

7.5Translational Control

7.5.1Global Control

7.5.2mRNA-Specific Control

7.6mRNA Localization

7.7Protein Regulation

7.8Experiments

7.8.1Beads-on-a-string Structure

7.8.2Repression of Gene Expression in Heterochromatin

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 8 DNA Replication

8.1Semi-Conservative Replication

8.2Initiation of Replication

8.3Semi-Discontinuous Replication

8.4Elongation of Replication and its Proteins

8.4.1Helicase and SSBs

8.4.2DNA Polymerases

8.4.3Explanation for 3′→5′Synthesis

8.4.4Primers

8.5DNA Topology

8.6DNA Replication in Eukaryotes

8.6.1Initiation of DNA Replication in Eukaryotes

8.6.2Telomeres

8.7Experiments

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 9 Mutations and Mutation Repair

9.1DNA Damage and Mutations

9.2Point Mutations

9.2.1Mismatched Base

9.2.2Spontaneous Mutations

9.2.3Induced Mutations

9.3Insertions and Deletions

9.3.1Strand Slippage

9.3.2Transposons

9.3.3Intercalating Agents

9.4Large-Scale DNA Changes

9.5Consequences of DNA Mutations

9.5.1Consequences of Point Mutations

9.5.2Consequences of Insertions and Deletions

9.5.3Consequences of Translocations

9.5.4Mutation Hot Spots

9.6Mutation Repair

9.6.1Direct Reversal

9.6.2Mismatch Repair

9.6.3Nucleotide Excision Repair

9.6.4Base Excision Repair

9.6.5Double-Stranded Break Repair

9.7Experiments

9.7.1Nucleotide Excision Repair and Human Disease

9.7.2The Ames Test

Summary

Vocabulary

Review Questions

Exploration Questions



Chapter 10 Recombination

10.1Homologous Recombination

10.1.1Mechanism for Crossing-Over

10.1.2Mechanism for Double-Stranded Break Repair

10.1.3The RecBCD Pathway

10.1.4Gene Conversion

10.2Non-homologous Recombination

10.2.1Transposons

10.2.2Retrotransposons

10.2.3Bacteriophage λ Integration

Summary

Vocabulary

Review Questions

Exploration Questions



Answers to Review Questions

Index(Chinese)

Index(English)





绪论

第1章从氨基酸到蛋白质

1.1蛋白质的组成

1.2蛋白质的构象

1.2.1描述蛋白质的结构

1.2.2蛋白质折叠的化学和物理基础

1.3蛋白质的结构与功能：几个例子

1.4蛋白质动力学

1.5实验研究

小结

词汇

习题

思考题



第2章核酸

2.1遗传物质的性质

2.2核酸与DNA结构

2.2.1核苷酸

2.2.2核酸的一般结构

2.2.3DNA的结构

2.3DNA作为遗传物质

2.4细胞中的DNA

2.5RNA（核糖核酸）

2.6实验研究

小结

词汇

习题

思考题



第3章原核生物转录：机理与调控

3.1为什么使用RNA作为中间物

3.2转录机理

3.2.1启动子

3.2.2RNA聚合酶

3.2.3转录机理的三个阶段

3.3原核生物基因表达调控

3.3.1协同调控

3.3.2乳糖操纵子

3.3.3色氨酸操纵子

3.3.4阿拉伯糖与半乳糖操纵子

3.4实验研究

小结

词汇

习题

思考题



第4章真核生物转录：机理与调控

4.1真核生物RNA聚合酶

4.2真核生物启动子

4.3通用转录因子与转录起始

4.3.1TFⅡD

4.3.2其他TFⅡs

4.3.3RNA聚合酶Ⅰ和Ⅲ的通用转录因子

4.4特异转录因子与转录调控

4.4.1激活蛋白

4.4.2阻遏蛋白

4.4.3增强子和沉默子

4.5特异转录因子的结构

4.5.1原核生物DNA结合基序

4.5.2真核生物DNA结合基序

4.6实验研究

4.6.1RNA聚合酶的目标

4.6.2特异转录因子的模块化Summary

词汇

习题

思考题



第5章真核生物mRNA的修饰

5.1加帽

5.2聚腺苷酸化

5.3剪接

5.3.1基本的剪接反应

5.3.2在剪接中发挥作用的蛋白质

5.3.3自我剪接

5.3.4反式剪接

5.3.5内含子存在的原因

5.4mRNA编辑

5.5实验研究

小结

词汇

习题

思考题



第6章翻译

6.1遗传密码

6.2原核生物翻译机理

6.2.1起始

6.2.2延伸

6.2.3终止

6.3真核生物翻译

6.4tRNA结构与摇摆

6.4.1反密码子

6.4.2摇摆

6.5实验研究

6.5.1破译遗传密码

6.5.2翻译的方向

小结

词汇

习题

思考题



第7章真核生物基因表达调控

7.1组蛋白与转录调控

7.1.1组蛋白与DNA组织

7.1.2组蛋白与转录

7.1.3组蛋白的共价修饰

7.1.4识别和修饰组蛋白的蛋白质

7.2转录后调控

7.3细胞核输出

7.4RNA稳定性

7.4.1蛋白质调控mRNA稳定性

7.4.2小RNA调控mRNA稳定性

7.5翻译调控

7.5.1全局控制

7.5.2mRNA特异性控制

7.6mRNA定位

7.7蛋白质调控

7.8实验研究

7.8.1线珠结构

7.8.2异染色质中基因表达的阻遏

小结

词汇

习题

思考题



第8章DNA复制

8.1半保留复制

8.2复制的起始

8.3半不连续复制

8.4复制延伸及其相关蛋白

8.4.1解旋酶与SSB

8.4.2DNA聚合酶

8.4.3关于3′→5′合成

8.4.4引物

8.5DNA拓扑学

8.6真核生物DNA复制

8.6.1真核生物DNA复制起始

8.6.2端粒

8.7实验研究

小结

词汇

习题

思考题



第9章突变与突变修复

9.1DNA损伤与突变

9.2点突变

9.2.1错配的碱基

9.2.2自发突变

9.2.3诱发突变

9.3插入和缺失

9.3.1链滑动

9.3.2转座子

9.3.3嵌入剂

9.4大规模DNA变化

9.5DNA突变的后果

9.5.1点突变的后果

9.5.2插入和缺失的后果

9.5.3易位的后果

9.5.4突变热点

9.6突变修复

9.6.1直接回复

9.6.2错配修复

9.6.3核苷酸切除修复

9.6.4碱基切除修复

9.6.5双链断裂修复

9.7实验研究

9.7.1核苷酸切除修复与人类疾病

9.7.2埃姆斯测验法

小结

词汇

习题

思考题



第10章重组

10.1同源重组

10.1.1交换机理

10.1.2双链断裂修复机理

10.1.3RecBCD途径

10.1.4基因转换

10.2非同源重组

10.2.1转座子

10.2.2反转录转座子

10.2.3λ噬菌体的整合

小结

词汇

习题

思考题



习题答案

中文索引

英文索引

参考文献