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ISBN：9780121647308

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　　"4 Stars - These 4 volumes provide a modern, near encyclopedic review of cell biology protocols." --Gregory Jay Bix, Thomas Jefferson University in DOODY'S (May 2006) 　　　　　　 "4 Stars - These 4 volumes provide a modern, near encyclopedic review of cell biology protocols." 　　--Gregory Jay Bix, Thomas Jefferson University in DOODY'S (May 2006) 　　　　　　 "The aim of Cell Biology: A Laboratory Handbook is to assemble in one place the most important methods in cell biology, which have withstood the test of time to become classics. This goal has been admirably achieved in a three-volume set edited by Julio E. Celis. This Handbook is divided into 15 sections, starting with techniques for culturing and analyzing whole cells, progressing through sections on cell fractionation, microscopy, generation of antibodies, introduction of molecules into living cells, and ending up with methods for analysis of proteins. Chapters are short and to the point. Methods are described in sufficient detail to allow the techniques to be reproduced, and are provided as easy-to-follow recipes. Importantly, the pitfalls inherent in each technique are assembled at the end of each article. The layout is admirably clear, and the quality of the illustrations is superb. The compendium will undoubtedly prove invaluable to cell biologists and to molecular biologists who wish to study the role of macromolecules in cell function. Cell Biology: A Laboratory Handbook should become the bible of established cell biological methods." (--Tony Hunter, Salk Institute, La Jolla, California) 　　 This Second Edition of the highly praised Cell Biology: A Laboratory Handbook brings together 260 new and revised chapters. Each chapter is concisely written and beautifully illustrated, making this attractive four-volume set a worthwhile addition to any desktop, and the up-to-date instructions for biological techniques make this reference the next best thing to having the expert at your side. Dr. Julio Celis and the Associate Editors have drawn on peer review from the scientific community to include 40 percent new material in this much-needed and updated laboratory manual. In one easy to use reference, current and classic protocols are presented in a clear and reader-friendly format that makes this manual a necessity to undergraduate and graduate students as well as technicians and instructors. --This text refers to the Plastic Comb edition. 　　　　　　 "I look forward to the publication of the Handbook with great interest. There is clearly an enormous need for such a book, and I think the cell biology community--and my students--will be grateful." --This text refers to the Plastic Comb edition.　　

Volume 1
Part A Cell and Tissue Culture: Associated Techniques
Section 1 General Techniques
Section 2 Culture of Specific Cell Types: Stem Cells
Section 3 Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial
Section 4 Differentiation and Reprogramming of Somatic Cells
Section 5 Immortalisation
Section 6 Somatic Cell Hybrids
Section 7 Cell Separation Techniques
Section 8 Cell Cycle Analysis
Section 9 Cytotoxic and Cell Growth Assays
Section 10 Apoptosis
Section 11 Assays of Cell Transformation, Tumorigenesis, Invasion and Wound Healing
Section 12 Electrophysiological Methods
Section 13 Organ Cultures
Part B Viruses
Section 14 Growth and Purification of Viruses
Part C Antibodies
Section 15 Production and Purification of Antibodies
Part D Immunocytochemistry
Section 16 Immunofluoresence
Part E Appendix
Volume 2
Part A Organelles and Cellular Structures
Section 1 Isolation: Plasma Membrane, Organelles, and Cellular Structures
Section 2 Vital Staining of Cells/Organelles
Section 3 Protein Purification
Part B Assays
Section 4 Endocytic and Exocytic Pathways
Section 5 Membranes
Section 6 Mitochondria
Section 7 Nuclear Transport
Section 8 Chromatin Assembly
Section 9 Signal Transduction Assays
Section 10 Assays and Models of in Vitro and in Vitro Motility
Section 11 Mechanical Stress in Single Cells
Part C Appendix
Volume 3
Part A Imaging Techniques
Section 1 Light Microscopy
Section 2 Digital Video Microscopy
Section 3 Confocal Microscopy of Living Cells and Fixed Cells
Section 4 Fluorescent Microscopy of Living Cells
Section 5 Use of Fluorescent Dyes for Studies of Intracellular Physiological Parameters
Section 6 Digital Image Processing, Analysis, Storage, and Display
Part B
Section 7
Section 8
Section 9
Section 10
Part C
Section 11
Part D
Section 12
Part E
Section 13
Part F
Section 14
Section 15
Part G
Section 16
Part H
Section 17
Volume 4
Part A
Section 1
Section 2
Section 3
Part B
Section 4
Part C
Section 5
Part D
Section 6
Section 7
Section 8
Section 9
Section 10
Section 11
Section 12
Section 13
Section 14
Section 15
Part E

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