Pocket guide to biotechnology and genetic engineering /

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作   者:Rolf D. Schmid ; 142 color plates by Ruth Hammelehle.

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ISBN:9783527308958

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简介

"Biotechnology and genetic engineering are the key technologies of the 21st century. They allow the findings in cell biology and genetics biochemistry and microbiology, biochemical engineering and bioinformatics to be applied to health care, agriculture, food production, environmental protection and alternative production methods for chemicals." "This pocket guide provides a broad overview of the relevant facts on products, methods and applications, while discussing the opportunities and risks of these new technologies as well as ethical, economic and safety considerations. The whole is backed by instructive yet attractive color illustrations and an excellent didactic approach throughout." "A perfect introduction to the field - for professionals and students alike."--BOOK JACKET.

目录

Preface p. IX
Introduction p. 1
Historical survey
Early developments p. 2
Biotechnology today p. 4
Food biotechnology
Alcoholic beverages p. 6
Beer p. 8
Fermented food p. 10
Food and lactic acid fermentation p. 12
Alcohols, acids, and amino acids Ethanol p. 14
1-Butanol, acetone p. 16
Acetic acid/vinegar p. 18
Citric acid p. 20
Lactic acid, gluconic acid p. 22
Amino acids p. 24
L-Glutamic acid p. 26
D,L-Methionine, L-lysine, and L-threonine p. 28
Aspartame, L-phenylalanine, and L-aspartic acid p. 30
Amino acids via enzymatic transformation p. 32
Antibiotics
Antibiotics: occurrence, applications, mechanism of action p. 34
Antibiotics: industrial production, resistance p. 36
[beta]-Lactam antibiotics: structure, biosynthesis and mechanism of action p. 38
[beta]-Lactam antibiotics: manufacture p. 40
Amino acid and peptide antibiotics p. 42
Glycopeptide, polyether, and nucleoside antibiotics p. 44
Aminoglycoside antibiotics p. 46
Tetracyclines, chinones, chinolones, and other aromatic antibiotics p. 48
Macrolide antibiotics p. 50
New pathways to antibiotics p. 52
Specialties
Vitamins p. 54
Nucleosides and nucleotides p. 56
Biosurfactants and biocosmetics p. 58
Microbial polysaccharides p. 60
Biomaterials p. 62
Biotransformation p. 64
Steroid biotransformations p. 66
Enzymes
Enzymes p. 68
Enzyme catalysis p. 70
Analytical enzymes p. 72
Enzyme tests p. 74
Enzymes as additives p. 76
Detergent enzymes p. 78
Enzymes for starch hydrolysis p. 80
Enzymatic starch hydrolysis p. 82
Enzymes and sweeteners p. 84
Enzymes for the hydrolysis of cellulose and polyoses p. 86
Enzymes in pulp and paper processing p. 88
Pectinases p. 90
Enzymes and milk products p. 92
Enzymes in baking and meat processing p. 94
Enzymes in leather and textile treatment p. 96
Procedures for obtaining novel technical enzymes p. 98
Bakers' yeast and single cell technologies
Bakers' yeast and fodder yeasts p. 100
Single cell protein, single cell oil p. 102
Biotechnology and environmental processes
Aerobic wastewater treatment p. 104
Anaerobic wastewater and sludge treatment p. 106
Biological treatment of exhaust air p. 108
Biological soil treatment p. 110
Microbial leaching, biofilms, and biocorrosion p. 112
Medical biotechnology
Insulin p. 114
Growth hormone and other hormones p. 116
Hemoglobin, serum albumen, and lactoferrin p. 118
Blood clotting agents p. 120
Anticoagulants and thrombolytic agents p. 122
Enzyme inhibitors p. 124
The immune system p. 126
Stem cells p. 128
Tissue Engineering p. 130
Interferons p. 132
Interleukins p. 134
Erythropoietin and other growth factors p. 136
Other therapeutic proteins p. 138
Vaccines p. 140
Recombinant vaccines p. 142
Antibodies p. 144
Monoclonal antibodies p. 146
Recombinant and catalytic antibodies p. 148
Immunoanalysis p. 150
Biosensors p. 152
Agricultural biotechnology
Animal breeding p. 154
Embryo transfer, cloned animals p. 156
Gene maps p. 158
Transgenic animals p. 160
Gene farming and xenotransplantation p. 162
Plant breeding p. 164
Plant tissue surface culture p. 166
Plant cell suspension culture p. 168
Transgenic plants: methods p. 170
Transgenic plants: resistance p. 172
Transgenic plants: products p. 174
Microbiological fundamentals
Viruses p. 176
Bacteriophages p. 178
Microorganisms p. 180
Bacteria p. 182
Some bacteria of importance for biotechnology p. 184
Fungi p. 186
Yeasts p. 188
Microorganisms: isolation, preservation, safety p. 190
Microorganisms: strain improvement p. 192
Bioengineering fundamentals
Growing microorganisms p. 194
Growth kinetics and product formation p. 196
Fed-batch and continuous fermentation p. 198
Fermentation technology p. 200
Fermentation technology: scale-up p. 202
Cultivation of mammalian cells p. 204
Mammalian cell bioreactors p. 206
Enzyme and cell reactors p. 208
Recovery of bioproducts p. 210
Recovery of proteins: chromatography p. 212
Economic aspects of industrial processes p. 214
Fundamentals of molecular genetics
DNA: structure p. 216
DNA: function p. 218
Genetic engineering: general steps p. 220
Preparation of DNA p. 222
Other useful enzymes for DNA manipulation p. 224
PCR: general method p. 226
PCR: laboratory methods p. 228
DNA: synthesis and size determination p. 230
DNA sequencing p. 232
Transfer of foreign DNA in living cells (transformation) p. 234
Gene cloning and identification p. 236
Gene expression p. 238
Gene silencing p. 240
RNA p. 242
Gene libraries and gene mapping p. 244
Genetic maps of prokaryotes p. 246
Genetic maps of eukaryotes p. 248
The human genome p. 250
Functional analysis of the human genome p. 252
Recent trends
DNA assays p. 254
DNA and protein arrays p. 256
Reporter groups p. 258
Protein design p. 260
Gene therapy p. 262
Proteomics p. 264
Drug screening p. 266
Bioinformatics p. 268
Metabolism p. 270
Metabolic engineering p. 272
Systems biology p. 274
Safety, ethics, economic issues
Safety in genetic engineering p. 276
Regulation of products derived from biotechnology p. 278
Ethical considerations and acceptance p. 280
Patents in biotechnology p. 282
International aspects of biotechnology p. 284
Index p. 287
Literature p. 319
Origin of materials p. 345

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