简介
Summary:
Publisher Summary 1
This is the second volume of a two-volume laboratory procedures reference (the first is on isolation and characterization models) concerning a type of investigation that holds high promise (if politics don't impede progress). Each article introduces a protocol, details the necessary materials and methods, and provides notes. Contributors are researchers in genetics and related biomedical disciplines from many industrialized countries. The included disk contains illustrations. Annotation 漏2006 Book News, Inc., Portland, OR (booknews.com)
Publisher Summary 2
It is clear that the potentials of assessing embryonic stem (ES) cells in regenerative medicine applications is evident in the ever-increasing publications in which ES cell biology and differentiation along diverse lineages appear in the academic as well as the popular press. These two new volumes present important advances in the field since the publication of Embryonic Stem Cells: Methods and Protocols four years ago. These two volumes provide an update and complement to that volume, focusing on ES cells recently isolated from other/non-mouse species. Each volume contains numerous updates, more advanced approaches, and completely new protocols for the use of ES cells in studies of diverse cell lineages. These two volumes will surely expand the experimental repertoires of both experts and novices in the field.
Publisher Summary 3
Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Protocols provides a diverse collection of readily reproducible cellular and molecular protocols for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Protocols: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Protocols: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular protocols for the isolation, maintenance, and characterization of embryonic stem cells.聽Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.
目录
Preface p. v
Contents of the Companion Volume p. xi
Contributors p. xv
Guide to the Companion CD p. xxi
Part I Isolation and Maintenance
1 Isolation and Differentiation of Medaka Embryonic Stem Cells Yunhan Hong and Manfred Schartl p. 3
2 Maintenance of Chicken Embryonic Stem Cells In Vitro Hiroyuki Horiuchi and Shuichi Furusawa and Haruo Matsuda p. 17
3 Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro Satoshi Tanaka p. 35
4 Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro Maren Schulze and Hendrik Ungefroren and Michael Bader and Fred F盲ndrich p. 45
5 Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells Shigeo Saito and Ken Sawai and Arika Minamihashi and Hideyo Ugai and Takehide Murata and Kazunari K. Yokoyama p. 59
6 Generation and Characterization of Monkey Embryonic Stem Cells Hirofumi Suemori and Norio Nakatsuji p. 81
7 Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture Jennifer Nichols and Qi-Long Ying p. 91
Part II Signaling in Embryonic Stem Cell Differentiation
8 Internal Standards in Differentiating Embryonic Stem Cells In Vitro Christopher L. Murphy p. 101
9 Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development With Cultured Embryonic Stem Cells Shaohua Li and Peter D. Yurchenco p. 113
10 Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells Leo R. Quinlan p. 127
11 Cripto Signaling in Differentiating Embryonic Stem Cells Gabriella Minchiotti and Silvia Parisi and M. Graziella Persico p. 151
12 The Use of Embryonic Stem Cells to Study Hedgehog Signaling Sandy Becker and Laura Grabel p. 171
13 Transfection and Promoter Analysis in Embryonic Stem Cells Sangmi Chung and Kwang-Soo Kim p. 187
14 SAGE Analysis to Identify Embryonic Stem Cell-Predominant Transcripts Kenneth R. Boheler and Kirill V. Tarasov p. 195
15 Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4 Yoshimi Tokuzawa and Masayoshi Maruyama and Shinya Yamanaka p. 223
16 Gene Silencing Using RNA Interference in Embryonic Stem Cells J. Matthew Velkey and Nicole A. Slawny and Theresa E. Gratsch and K. Sue O'Shea p. 233
Part III Genetic Manipulation of Embryonic Stem Cells
17 Efficient Transfer of HSV-1 Amplicon Vectors Into Embryonic Stem Cells and Their Derivatives Dieter Riethmacher and Filip Lim and Thomas Schimmang p. 265
18 Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells Masahiro Oka and Lung-Ji Chang and Frank Costantini and Naohiro Terada p. 273
19 Use of the Cytomegalovirus Promoter for Transient and Stable Transgene Expressionin Mouse Embryonic Stem Cells Katie M. Barrow and Flor M. Perez-Campo and Christopher M. Ward p. 283
20 Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells Takayuki Asano and Hiroaki Shibata and Yutaka Hanazono p. 295
21 Generation of Green Fluorescent Protein-Expressing Monkey Embryonic Stem Cells Tatsuyuki Takada and Yutaka Suzuki and Nae Kadota and Yasushi Kondo and Ryuzo Torii p. 305
22 DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells Yiling Hong and Rachel B. Cervantes and Peter J. Stambrook p. 313
23 Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro Dakang Xu and Trevor J. Wilson and Paul J. Hertzog p. 327
Part IV Use of Embryonic Stem Cells in Pharmacological and Toxicological Screens
24 Use of Differentiating Embryonic Stem Cells in Pharmacological Studies Brigitte Wdziekonski and Phi Villageois and C茅cile Vernochet and Blaine Phillips and Christian Dani p. 341
25 Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens Patrick J. Mee and Carmel M. O'Brien and Hazel Thomson and Sjaak van der Sar and Viktor Lakics and Timothy E. Allsopp p. 353
26 Use of Murine Embryonic Stem Cells in Embryotoxicity Assays: The Embryonic Stem Cell Test Andrea E. M. Seiler and Roland Buesen and Anke Visan and Horst Spielmann p. 371
27 Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells Sonja Becker and Martin Hrab茅 de Angelis and Johannes Beckers p. 397
Part V Epigenetic Analysis of Embryonic Stem Cells
28 Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion Masako Tada and Takashi Tada p. 411
29 Methylation in Embryonic Stem Cells In Vitro Koichiro Nishino and Jun Ohgane and Masako Suzuki and Naka Hattori and Kunio Shiota p. 421
Part VI Tumor-Like Properties
30 Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells Kazutoshi Takahashi and Tomoko Ichisaka and Shinya Yamanaka p. 449
31 In Vivo Tumor Formation From Primate Embryonic Stem Cells Takayuki Asano and Kyoko Sasaki and Yoshihiro Kitano and Keiji Terao and Yutaka Hanazono p. 459
Part VII Animal Models and Therapy
32 Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Therapy Adeline A. Lau and Kim M. Hemsley and Adrian Meedeniya and Aaron J. Robinson and John J. Hopwood p. 471
33 Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model Fumihiko Nishimura and Hayato Toriumi and Shigeaki Ishizaka and Toshisuke Sakaki and Masahide Yoshikawa p. 485
Index p. 495
Contents of the Companion Volume p. xi
Contributors p. xv
Guide to the Companion CD p. xxi
Part I Isolation and Maintenance
1 Isolation and Differentiation of Medaka Embryonic Stem Cells Yunhan Hong and Manfred Schartl p. 3
2 Maintenance of Chicken Embryonic Stem Cells In Vitro Hiroyuki Horiuchi and Shuichi Furusawa and Haruo Matsuda p. 17
3 Derivation and Culture of Mouse Trophoblast Stem Cells In Vitro Satoshi Tanaka p. 35
4 Derivation, Maintenance, and Characterization of Rat Embryonic Stem Cells In Vitro Maren Schulze and Hendrik Ungefroren and Michael Bader and Fred F盲ndrich p. 45
5 Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cells Shigeo Saito and Ken Sawai and Arika Minamihashi and Hideyo Ugai and Takehide Murata and Kazunari K. Yokoyama p. 59
6 Generation and Characterization of Monkey Embryonic Stem Cells Hirofumi Suemori and Norio Nakatsuji p. 81
7 Derivation and Propagation of Embryonic Stem Cells in Serum- and Feeder-Free Culture Jennifer Nichols and Qi-Long Ying p. 91
Part II Signaling in Embryonic Stem Cell Differentiation
8 Internal Standards in Differentiating Embryonic Stem Cells In Vitro Christopher L. Murphy p. 101
9 Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development With Cultured Embryonic Stem Cells Shaohua Li and Peter D. Yurchenco p. 113
10 Phosphoinositides, Inositol Phosphates, and Phospholipase C in Embryonic Stem Cells Leo R. Quinlan p. 127
11 Cripto Signaling in Differentiating Embryonic Stem Cells Gabriella Minchiotti and Silvia Parisi and M. Graziella Persico p. 151
12 The Use of Embryonic Stem Cells to Study Hedgehog Signaling Sandy Becker and Laura Grabel p. 171
13 Transfection and Promoter Analysis in Embryonic Stem Cells Sangmi Chung and Kwang-Soo Kim p. 187
14 SAGE Analysis to Identify Embryonic Stem Cell-Predominant Transcripts Kenneth R. Boheler and Kirill V. Tarasov p. 195
15 Utilization of Digital Differential Display to Identify Novel Targets of Oct3/4 Yoshimi Tokuzawa and Masayoshi Maruyama and Shinya Yamanaka p. 223
16 Gene Silencing Using RNA Interference in Embryonic Stem Cells J. Matthew Velkey and Nicole A. Slawny and Theresa E. Gratsch and K. Sue O'Shea p. 233
Part III Genetic Manipulation of Embryonic Stem Cells
17 Efficient Transfer of HSV-1 Amplicon Vectors Into Embryonic Stem Cells and Their Derivatives Dieter Riethmacher and Filip Lim and Thomas Schimmang p. 265
18 Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells Masahiro Oka and Lung-Ji Chang and Frank Costantini and Naohiro Terada p. 273
19 Use of the Cytomegalovirus Promoter for Transient and Stable Transgene Expressionin Mouse Embryonic Stem Cells Katie M. Barrow and Flor M. Perez-Campo and Christopher M. Ward p. 283
20 Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells Takayuki Asano and Hiroaki Shibata and Yutaka Hanazono p. 295
21 Generation of Green Fluorescent Protein-Expressing Monkey Embryonic Stem Cells Tatsuyuki Takada and Yutaka Suzuki and Nae Kadota and Yasushi Kondo and Ryuzo Torii p. 305
22 DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells Yiling Hong and Rachel B. Cervantes and Peter J. Stambrook p. 313
23 Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro Dakang Xu and Trevor J. Wilson and Paul J. Hertzog p. 327
Part IV Use of Embryonic Stem Cells in Pharmacological and Toxicological Screens
24 Use of Differentiating Embryonic Stem Cells in Pharmacological Studies Brigitte Wdziekonski and Phi Villageois and C茅cile Vernochet and Blaine Phillips and Christian Dani p. 341
25 Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens Patrick J. Mee and Carmel M. O'Brien and Hazel Thomson and Sjaak van der Sar and Viktor Lakics and Timothy E. Allsopp p. 353
26 Use of Murine Embryonic Stem Cells in Embryotoxicity Assays: The Embryonic Stem Cell Test Andrea E. M. Seiler and Roland Buesen and Anke Visan and Horst Spielmann p. 371
27 Use of Chemical Mutagenesis in Mouse Embryonic Stem Cells Sonja Becker and Martin Hrab茅 de Angelis and Johannes Beckers p. 397
Part V Epigenetic Analysis of Embryonic Stem Cells
28 Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion Masako Tada and Takashi Tada p. 411
29 Methylation in Embryonic Stem Cells In Vitro Koichiro Nishino and Jun Ohgane and Masako Suzuki and Naka Hattori and Kunio Shiota p. 421
Part VI Tumor-Like Properties
30 Identification of Genes Involved in Tumor-Like Properties of Embryonic Stem Cells Kazutoshi Takahashi and Tomoko Ichisaka and Shinya Yamanaka p. 449
31 In Vivo Tumor Formation From Primate Embryonic Stem Cells Takayuki Asano and Kyoko Sasaki and Yoshihiro Kitano and Keiji Terao and Yutaka Hanazono p. 459
Part VII Animal Models and Therapy
32 Directed Differentiation and Characterization of Genetically Modified Embryonic Stem Cells for Therapy Adeline A. Lau and Kim M. Hemsley and Adrian Meedeniya and Aaron J. Robinson and John J. Hopwood p. 471
33 Use of Differentiating Embryonic Stem Cells in the Parkinsonian Mouse Model Fumihiko Nishimura and Hayato Toriumi and Shigeaki Ishizaka and Toshisuke Sakaki and Masahide Yoshikawa p. 485
Index p. 495
- 名称
- 类型
- 大小
光盘服务联系方式: 020-38250260 客服QQ：4006604884
云图客服：
用户发送的提问，这种方式就需要有位在线客服来回答用户的问题，这种 就属于对话式的,问题是这种提问是否需要用户登录才能提问
Video Player
×
Audio Player
×
pdf Player
×
亲爱的云图用户，
光盘内的文件都可以直接点击浏览哦
无需下载，在线查阅资料！
