Regulation of gene expression : molecular mechanisms /
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作 者：by Gary H. Perdew, John P. Vanden Heuvel, Jeffrey M. Peters.
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ISBN：9781588292650
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简介
"Regulation of Gene Expression: Molecular Mechanisms presents a comprehensive overview of methods and approaches for characterizing mechanisms of gene regulation. The text is appropriate both as a graduate textbook and a standard laboratory reference and provides the essential groundwork for an advanced understanding of the various mechanisms that may result in altered activityof a specific cell protein." "Each of three sections explores mechanisms of gene regulation and expression, andpresents methods and protocols for achieving specific experimental goals. Part I focuses on approaches for studying control of mRNA expression and determining targetgenes for a given transcription copy. Part II outlines themethods for determining how proteins can regulate each other by mediating synthesis, degradation, protein-proteininteractions, and posttranslational modification. Part IIIexplores how gene targeting techniques in mice can provideinsight into protein function. This volume provides a clear, concise overview of the protocols and techniques used to examine chemically or disease-mediate alterations in gene expression in mammalian systems."--BOOK JACKET
目录
Preface p. v
Companion CD p. ix
Part I Gene Expression Control at the mRNA Level John P. Vanden Heuvel, PhD p. 1
Chapter 1 Overview p. 3
Chapter 2 Messenger RNA Accumulation p. 11
Chapter 3 Transcript Profiling p. 31
Chapter 4 Transcriptional Regulation of Gene Expression p. 49
Chapter 5 Posttranscriptional Processing of Messenger RNA p. 105
Study Questions p. 141
References p. 143
Part II Regulation of Protein Levels and Transcription Factor Function Gary H. Perdew, PhD p. 147
Contents p. 147
Chapter 1 Overview p. 149
Chapter 2 Protein Synthesis and Turnover p. 155
Chapter 3 Proteomics p. 161
Chapter 4 Determination of Protein-Protein Interactions and the Motifs That Mediate Them p. 173
Chapter 5 Posttranslational Modifications p. 215
Chapter 6 Dissection of Signaling Pathways p. 239
Study Questions p. 259
References p. 261
Part III Use of Transgenic and Knockout Mice to Study Gene Regulation Jeffrey M. Peters, PhD p. 265
Contents p. 265
Chapter 1 Overview p. 267
Chapter 2 Production of Transgenic Animals p. 271
Chapter 3 Knockout Animals p. 295
Chapter 4 Isolation of Cells and Cell Lines From Transgenic and Knockout Animals p. 319
Study Questions p. 321
References p. 323
Index p. 329
Companion CD p. ix
Part I Gene Expression Control at the mRNA Level John P. Vanden Heuvel, PhD p. 1
Chapter 1 Overview p. 3
Chapter 2 Messenger RNA Accumulation p. 11
Chapter 3 Transcript Profiling p. 31
Chapter 4 Transcriptional Regulation of Gene Expression p. 49
Chapter 5 Posttranscriptional Processing of Messenger RNA p. 105
Study Questions p. 141
References p. 143
Part II Regulation of Protein Levels and Transcription Factor Function Gary H. Perdew, PhD p. 147
Contents p. 147
Chapter 1 Overview p. 149
Chapter 2 Protein Synthesis and Turnover p. 155
Chapter 3 Proteomics p. 161
Chapter 4 Determination of Protein-Protein Interactions and the Motifs That Mediate Them p. 173
Chapter 5 Posttranslational Modifications p. 215
Chapter 6 Dissection of Signaling Pathways p. 239
Study Questions p. 259
References p. 261
Part III Use of Transgenic and Knockout Mice to Study Gene Regulation Jeffrey M. Peters, PhD p. 265
Contents p. 265
Chapter 1 Overview p. 267
Chapter 2 Production of Transgenic Animals p. 271
Chapter 3 Knockout Animals p. 295
Chapter 4 Isolation of Cells and Cell Lines From Transgenic and Knockout Animals p. 319
Study Questions p. 321
References p. 323
Index p. 329
Regulation of gene expression : molecular mechanisms /
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